EFFECTS OF OFLOXACIN ADMINISTRATION ON THE RELIABILITY OF URINE GLUCOSE TESTING

OBJECTIVE: To study the effects of ofloxacin on the reliability of uri ne glucose testing. DESIGN: Open-label, nonrandomized. SETTING: A univ ersity-affiliated tertiary care hospital, ambulatory clinic. PARTICIPA NTS: Ten healthy volunteers (8 men and 2 women) aged 22-39 years. MAIN OUTCOME MEASURES: Phase I (in vitro) involved the addition of selecte d amounts of ofloxacin to a set of standard 50-mL urine samples prepar ed to simulate glycosuria. Phase II (in vivo) involved the oral admini stration of ofloxacin 400 mg to 10 subjects. Urine was collected: (1) immediately predose, (2) pooled 0-4 hours postdose, and (3) pooled 4-8 hours postdose, Known glucose concentrations were then added to these samples. Clinitest and Diastix tests were performed on all samples. T he accuracy of these tests in determining glucose concentrations was c ompared among urine samples taken before and after ofloxacin dosing. R ESULTS: None of the ofloxacin concentrations in phase I (0, 25, 50, 10 0, 200, 400, and 800 mu g/mL) influenced these testing methods at the urine glucose concentrations of 0.0%, 0.5%, 1%, and 2%. Likewise, the accuracy of these two tests was unaffected by ofloxacin administration in phase II. CONCLUSION: In single-dose administration, ofloxacin doe s not interfere with Clinitest or Diastix for determining urine glucos e concentrations.