A V-SNARE IMPLICATED IN INTRA-GOLGI TRANSPORT

We report the identification of a putative v-SNARE (GOS-28), localized primarily to transport vesicles at the terminal rims of Golgi stacks. In vitro, GOS-28, a Golgi SNARE of 28 kD, is efficiently packaged int o Golgi-derived vesicles, which are most likely COPI coated. Antibodie s directed against GOS-28 block its ability to bind alpha-SNAP, partia lly inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP-coated vesicles, but do accumulate docked unco ated vesicles.