Cyf. Huang et Je. Ferrell, DEPENDENCE OF MOS-INDUCED CDC2 ACTIVATION ON MAP KINASE FUNCTION IN ACELL-FREE SYSTEM, EMBO journal, 15(9), 1996, pp. 2169-2173
The progression of G(2)-arrested Xenopus laevis oocytes into meiotic M
-phase is accompanied by the nearly simultaneous activation of p42 MAP
kinase and Cdc2/cyclin B, This timing raises the possibility that the
activation of one kinase might depend upon the other, Here we have ex
amined whether Cdc2 activation requires p42 MAP kinase function. We ha
ve reconstituted Mos-induced Cdc2 activation in cell-free Xenopus oocy
te extracts, and have found that Mos-induced Cdc2 activation requires
active p42 MAP kinase, is inhibited by a MAP kinase phosphatase and is
independent of protein synthesis. These findings indicate that p42 MA
P kinase is an essential component of the M phase trigger in this syst
em.