THE HUMAN PLACENTA EXPRESSES TRANSCRIPTION ENHANCER FACTOR-I BUT THERE IS NO CORRELATION WITH THE EXPRESSION OF PLACENTAL-LACTOGEN

A transcriptional enhancer which has a consensus binding sequence for transcription enhancer factor-1 (TEF-1) has been found 3' of the hPL(3 ) gene. We examined whether TEF-1 is expressed by the human placenta a nd whether such expression is co-ordinated with that of human placenta l lactogen (hPL). Probing Northern blots of total RNA from first trime ster and term placenta, the choriocarcinoma-derived cell line JAr and primary cultured cytotrophoblast cells with a cDNA for TEF-1 revealed transcripts of 12-13 kb and 3-4 kb.The level of TEF-1 expression was t he same in first trimester as compared with term placenta and in undif ferentiated JAr as compared with differentiated cytotrophoblast cells. hPL expression was tenfold higher in term compared with first trimest er placenta and, whilst detectable in cytotrophoblast cells, was undet ectable in JAr cells. These data show that TEF-1 is expressed by the p lacenta but is not co-ordinated with hPL expression.