DISTINCT AREAS OF ALLELIC LOSS ON CHROMOSOMAL REGIONS 10P AND 10Q IN HUMAN PROSTATE-CANCER

Utilizing tissue microdissection and PCR techniques, we have examined 35 prostate tumors paired with normal tissues from the same patients f or allelic loss at 24 polymorphic loci spanning chromosome 10, Twenty- five tumors (71%) were deleted for at least one chromosome 10 locus, O f the total 35 tumors, 6 (17%) were deleted for 10p loci only, 5 (14%) for 10q loci only, and 14 (40%) were deleted for both 10p and 10q loc i, The common region of deletion on 10p included loci D10S211-D10S89-D 10S111. Fluorescence in situ hybridization of yeast artificial chromos ome probes encompassing these loci demonstrated that the 10p region of deletion maps to 10p11.2. Losses involving 10p loci alone were most c ommon in localized (5/14, 36%) and least common in metastatic (0/8) tu mors, The common region of deletion on 10q included loci D10S219-D10S2 15, consistent with the major region of deletion recently defined for prostate tumors on 10q. Losses involving 10q loci alone were lowest in localized and locally invasive tumors (1/14 and 2/15 respectively) an d highest in tumors metastatic to regional lymph nodes (2/8). These re sults suggest that 10p losses may define less invasive tumors, whereas 10q losses may play a role in the progression to more advanced tumor states in the prostate. Furthermore, this is the first report of allel ic loss of a defined region on 10p potentially harboring tumor suppres sor gene loci in human prostate cancer.