REDUCTION OF HUMAN-IMMUNODEFICIENCY-VIRUS PRODUCTION AND CYTOPATHIC EFFECTS BY INHIBITORS OF THE NA+ K+/2CL(-) COTRANSPORTER/

Infection of CD4(+) T-lymphoblastoid cells by cytopathic strains of HI V-1 results in an increase in cell volume that leads to lysis and cell death. The increase in volume is attributable in part to an HIV-induc ed increase in intracellular monovalent ion concentrations mediated by the plasma membrane-associated Na+/K+/2 Cl- cotransporter. Loop diure tics, which inhibit cotransporter activity, blocked HIV-induced HIV pr oduction and cytopathic effects at physiologically employed concentrat ions. (C) 1996 Academic Press, Inc.