Anaerobic digestion of dissolved, suspended and solid organics has rap
idly evolved in the last decades but nevertheless still faces several
scientific unknowns. In this review, some fundamentals of bacterial co
nversions and adhesion are addressed initially, It is argued in the li
ght of Delta G-values of reactions, and in view of the minimum energy
quantum per mel, that anaerobic syntrophs must have special survival s
trategies in order to support their existence: redistributing the avai
lable energy between the partners, reduced end-product fermentation re
actions and special cell-to-cell physiological interactions. In terms
of kinetics, it appears that both reaction rates and residual substrat
e thresholds are strongly related to minimum Delta G-values. These new
fundamental insights open perspectives for efficient design and opera
tion of anaerobic bioprocesses. Subsequently, an overview is given of
the current anaerobic biotechnology. For treating wastewaters, a novel
and high performance new system has been introduced during the last d
ecade: the upflow anaerobic sludge blanket system (UASB). This reactor
concept requires anaerobic consortia to grow in a dense and eco-physi
ologically well-organized way. The microbial principles of such granul
ar sludge growth are presented, Using a thermodynamic approach, the fo
rmation of different types of aggregates is explained. The application
of this bioprocess in worldwide wastewater treatment is indicated. Du
e to the long retention times of the active biomass, the UASB is also
suitable for the development of bacterial consortia capable of degradi
ng xenobiotics. Operating granular sludge reactors at high upflow velo
cities (5-6 m/h) in expanded granular sludge bed (EGSB) systems enlarg
es the application field to very low strength wastewaters (chemical ox
ygen demand < 1 g/l) and psychrophilic temperatures (10 degrees C). Fo
r the treatment of organic suspensions, there is currently a tendency
to evolve from the conventional mesophilic continuously stirred tank s
ystem to the thermophilic configuration, as the latter permits higher
conversion rates and easier sanitation. Integration of ultrafiltration
in anaerobic slurry digestion facilitates operation at higher volumet
ric loading rates and at shorter residence times. With respect to orga
nic solids, the recent trend in society towards source separated colle
ction of biowaste has opened a broad range of new application areas fo
r solid state anaerobic fermentation.