RAPID AND SIMPLE ASSAY FOR FERULOYL AND P-COUMAROYL ESTERASES

Authors
ONEILL FH CHRISTOV LP BOTES PJ PRIOR BA
Citation
Fh. Oneill et al., RAPID AND SIMPLE ASSAY FOR FERULOYL AND P-COUMAROYL ESTERASES, World journal of microbiology & biotechnology, 12(3), 1996, pp. 239-242
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
World journal of microbiology & biotechnology → ACNP
ISSN journal
09593993
Volume
12
Issue
3
Year of publication
1996
Pages
239 - 242
Database
ISI
SICI code
0959-3993(1996)12:3<239:RASAFF>2.0.ZU;2-X
Abstract
A rapid, simple and sensitive method for detection of ferulic and p-co umaric acids using HPLC has been developed which can be used to determ ine the respective phenolic acid esterase activities of microorganisms . Prior concentration, purification or derivatization of the samples a re not required. As little as 0.5 mg ferulic or p-coumaric acid/l coul d be detected and estimated in <1 h. The method is specific for the tw o phenolic acids since no interference by other components was observe d.