S. Ozkizilcik et Fle. Chu, PREPARATION AND CHARACTERIZATION OF A COMPLEX MICROENCAPSULATED DIET FOR STRIPED BASS MORONE-SAXATILIS LARVAE, Journal of microencapsulation, 13(3), 1996, pp. 331-343
Citations number
27
Categorie Soggetti
Pharmacology & Pharmacy","Chemistry Applied","Engineering, Chemical
The nutritional requirements of marine fish larvae are not well unders
tood, primarily due to the lack of biochemically defined diets accepta
ble by the larvae. This study describes the methodology for the prepar
ation and the characterization of a complex protein-walled microcapsul
e (CPWC) containing lipid-walled capsules (LWC). The CPWC were prepare
d by atomizing a mixture of casein, LWC and other dietary materials in
to a cyclohexane solution containing 1% (v/v) cross-linking reagent, a
dipoyl chloride. The primary purpose of complex microencapsulation was
to allow the gradual release of low molecular weight phagostimulants
and nutrients from the cross-linked protein wall. To determine the rel
ease kinetics of low molecular weight compounds from the complex diet,
a solution of the amino acid, lysine, was encapsulated in LWC and its
leaching rate from CPWC was assessed. A batch of conventional protein
-walled capsules (PWC) were also prepared by mixing lysine with casein
. Tile release of lysine from CPWC was significantly lower than that m
easured from conventional PWC (p < 0.05). Nearly 45% of the total lysi
ne leached out of freeze-dried PWC within 5 min of hydration, reaching
100% after 2 h. CPWC, on the other hand, efficiently retained encapsu
lated lysine, releasing only 25% after 2 h. In-vitro experiments indic
ated that CPWC were readily digested by the crude enzyme extract from
striped bass larvae and purified porcine pepsin and trypsin.