CONSTRUCTION OF SECRETION VECTORS FOR ESCHERICHIA-COLI

Nucleotide sequences coding for the signal peptides of three secreted proteins: alkaline phosphatase, outer membrane protein OmpA of Escheri chia coli, and protein A of Staphylococcus aureus were amplified by po lymerase chain reaction. Restriction sites for EcoRI and HindIII were introduced into the 5' and 3' ends of amplified sequences, respectivel y. Vectors with the signal peptide-coding sequences under the control of tip and tac promoters were constructed. Thre vectors enable cloning of genes for foreign proteins at the unique HindIII site. Plasmids fo r expression and secretion of human growth hormone were obtained.