ADMINISTRATION OF INTERLEUKIN-10 ABOLISHES INNATE RESISTANCE TO LISTERIA-MONOCYTOGENES

We have used severe-combined immunodeficient (SCID) mice resulted in s ecretion of interferon (IFN)-gamma from natural killer cells in vitro. This response was enhanced up to 15-fold in the presence of exogenous IL-2 but was completely ablated by addition of IL-10 with and IC50 of <0.5 U/ml. Infection of SCID mice with viable Listeria in vivo result ed in a prolonged course of infection eventually causing death by 12-1 4 days, whereas daily administration of IL-10 increased bacterial repl ication in the liver and spleen by up to 1000-fold resulting in death by day 4 post-infection. The immunosuppressive actions of IL-10 in viv o were also observed in immunocompetent BALB/c mice. where doses as lo w as 100 U/day converted a sublethal infection to 100% mortality. To s tudy the events controlling expression of endogenous IL-10. peritoneal macrophage monolayers were challenged with Listeria after pre-incubat ion with a panel of recombinant cytokines. IFN-gamma primed macrophage s for enhanced tumor necrosis factor (TNF) secretion. but inhibited IL -10 production, whereas granulocyte/macrophage colony-stimulating fact or (CSF). macrophage CSF and also IL-4 enhanced macrophage IL-10 respo nses after ingestion of Listeria in vitro. Finally, monoclonal antibod y neutralization of IFN-gamma during infection of SCID mice with Liste ria inhibited TNF-alpha mRNA. but augmented expression of IL-10 mRNA i n infected tissues. These results demonstrate that exogenous IL-10 is a potent immunosuppressive cytokine in the context of infection with a n intracellular bacterium and that expression of endogenous IL-10 vers us TNF is differentially regulated by the cytokine environment of the macrophage.