INTERLEUKIN-2 AND INTERLEUKIN-12 ACTIVATE NATURAL-KILLER CYTOLYTIC RESPONSES OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS FROM PATIENTS WITH HEAD AND NECK SQUAMOUS-CELL CARCINOMAE

Citation
Sp. Rashleigh et al., INTERLEUKIN-2 AND INTERLEUKIN-12 ACTIVATE NATURAL-KILLER CYTOLYTIC RESPONSES OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS FROM PATIENTS WITH HEAD AND NECK SQUAMOUS-CELL CARCINOMAE, Archives of otolaryngology, head & neck surgery, 122(5), 1996, pp. 541-547
Citations number
27
Categorie Soggetti
Otorhinolaryngology,Surgery
ISSN journal
08864470
Volume
122
Issue
5
Year of publication
1996
Pages
541 - 547
Database
ISI
SICI code
0886-4470(1996)122:5<541:IAIANC>2.0.ZU;2-X
Abstract
Objectives: To examine the capacity of interleukin-2 (IL-2) and interl eukin-12 (IL-12) to modulate the cytolytic activity of peripheral bloo d mononuclear cells against squamous cell carcinoma, and to determine whether peripheral blood mononuclear cells from healthy donors respond differently to IL-12 than do peripheral blood mononuclear cells from patients with head and neck squamous cell carcinoma. Design: Case-comp arison study of a consecutive sample of patients with head and neck sq uamous cell carcinoma who were scheduled to undergo surgical excision. Participants: The study included 10 patients with stage III or IV car cinoma matched with 10 volunteer blood donors. Intervention: Isolated peripheral blood mononuclear cells from patients and volunteers were t reated with IL-2, 10 U/mL and 100 U/mL; IL-12, 1 U/mL or 10 U/mL; or a combination of IL-2 and IL-12. Results: The combination of IL-2 and I L-12 consistently produced the greatest activation of cytolysis than e ither cytokine alone at all concentrations tested. This increased acti vity against a squamous cell carcinoma cell line was seen in lymphocyt es from volunteers and patients. Conclusion: Our findings suggest a ne w treatment regimen for the patient with head and neck cancer that use s immunomodulation with a combination of cytokines.