INTERLEUKIN-2 AND INTERLEUKIN-12 ACTIVATE NATURAL-KILLER CYTOLYTIC RESPONSES OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS FROM PATIENTS WITH HEAD AND NECK SQUAMOUS-CELL CARCINOMAE
Sp. Rashleigh et al., INTERLEUKIN-2 AND INTERLEUKIN-12 ACTIVATE NATURAL-KILLER CYTOLYTIC RESPONSES OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS FROM PATIENTS WITH HEAD AND NECK SQUAMOUS-CELL CARCINOMAE, Archives of otolaryngology, head & neck surgery, 122(5), 1996, pp. 541-547
Objectives: To examine the capacity of interleukin-2 (IL-2) and interl
eukin-12 (IL-12) to modulate the cytolytic activity of peripheral bloo
d mononuclear cells against squamous cell carcinoma, and to determine
whether peripheral blood mononuclear cells from healthy donors respond
differently to IL-12 than do peripheral blood mononuclear cells from
patients with head and neck squamous cell carcinoma. Design: Case-comp
arison study of a consecutive sample of patients with head and neck sq
uamous cell carcinoma who were scheduled to undergo surgical excision.
Participants: The study included 10 patients with stage III or IV car
cinoma matched with 10 volunteer blood donors. Intervention: Isolated
peripheral blood mononuclear cells from patients and volunteers were t
reated with IL-2, 10 U/mL and 100 U/mL; IL-12, 1 U/mL or 10 U/mL; or a
combination of IL-2 and IL-12. Results: The combination of IL-2 and I
L-12 consistently produced the greatest activation of cytolysis than e
ither cytokine alone at all concentrations tested. This increased acti
vity against a squamous cell carcinoma cell line was seen in lymphocyt
es from volunteers and patients. Conclusion: Our findings suggest a ne
w treatment regimen for the patient with head and neck cancer that use
s immunomodulation with a combination of cytokines.