P. Courtois et M. Pourtois, PURIFICATION OF NADH - HYPOTHIOCYANITE OXIDOREDUCTASE IN STREPTOCOCCUS-SANGUIS, Biochemical and molecular medicine, 57(2), 1996, pp. 134-138
NADH: hypothiocyanite oxidoreductase (NHOR) activity, found in some or
al Streptococci, is postulated to protect these microorganisms against
salivary peroxidase-produced hypothiocyanite, NHOR, however, has not
been purified so far. The purification of NHOR from crude extracts of
Streptococcus sanguis NCTC 7863 strain (by ultrafiltation and anion-ex
change chromatography) revealed one fraction of 125 +/- 1 kDa. However
, SDS-PAGE electrophoresis provided a single protein of 21.1 +/- 1.2 k
Da, This last discovery suggests that NHOR enzyme is a hexameric compl
ex having six subunits. (C) 1996 Academic Press, Inc.