Sh. Hu et al., THE 1.1 ANGSTROM CRYSTAL-STRUCTURE OF THE NEURONAL ACETYLCHOLINE-RECEPTOR ANTAGONIST, ALPHA-CONOTOXIN PNIA FROM CONUS-PENNACEUS, Structure, 4(4), 1996, pp. 417-423
Background: alpha-Conotoxins are peptide toxins, isolated from Conus s
nails, that block the nicotinic acetylcholine receptor (nAChR). The 16
-residue peptides PnlA and PnlB from Conus pennaceus incorporate the s
ame disulfide framework as other alpha-conotoxins but differ in functi
on from most alpha-conotoxins by blocking the neuronal nAChR, rather t
han the skeletal muscle subtype. The crystal structure determination o
f PnlA was undertaken to identify structural and surface features that
might be important for biological activity. Results: The 1.1 Angstrom
crystal structure of synthetic PnlA was determined by direct methods
using the Shake-and-Bake program. The three-dimensional structure inco
rporates a beta turn followed by two alpha-helical turns. The conforma
tion is stabilised by two disulfide bridges that form the interior of
the molecule, with all other side chains oriented outwards. Conclusion
s: The compact architecture of the PnlA toxin provides a rigid framewo
rk for presentation of chemical groups that are required for activity.
The structure is characterized by distinct hydrophobic and polar surf
aces; a 16 Angstrom separation of the sole positive and negative charg
es (these two charged residues being located at opposite ends of the m
olecule); a hydrophobic region and a protruding tyrosine side chain. T
hese features may be important for the specific interaction of PnlA wi
th neuronal nAChR.