NUCLEAR OXALATE BINDING-PROTEINS OF RAT-LIVER NUCLEI - A PRELIMINARY-REPORT

Authors
SELVAM R ANGAYARKANNI N VIJAYA R
Citation
R. Selvam et al., NUCLEAR OXALATE BINDING-PROTEINS OF RAT-LIVER NUCLEI - A PRELIMINARY-REPORT, Medical science research, 24(4), 1996, pp. 267-269
Citations number
16
Categorie Soggetti
Medicine, Research & Experimental
Journal title
Medical science research → ACNP
ISSN journal
02698951
Volume
24
Issue
4
Year of publication
1996
Pages
267 - 269
Database
ISI
SICI code
0269-8951(1996)24:4<267:NOBORN>2.0.ZU;2-E
Abstract
About three quarters of the rat total liver nuclear oxalate binding ac tivity is distributed in the nuclear membrane with a specific activity of 14.6 pmoles mg(-1) protein. Elution of Triton-X100 solubilised nuc lear membrane on Sephadex-G200 column yielded a protein containing bou nd [C-14]- oxalate with an approximate molecular weight of 68,000 Da. Although chromatin showed very little oxalate binding activity, isolat ed histones exhibited the highest specific activity (20 pmoles m(-1) p rotein).