THE ACRIDINE-ORANGE TEST - A CLINICALLY RELEVANT SCREENING METHOD FORSPERM QUALITY DURING INFERTILITY INVESTIGATION

To determine the clinical usefulness of Acridine Orange (AO) staining of spermatozoa as a screening test for the evaluation of semen quality during basic infertility investigation, semen smears from 103 randoml y chosen males of subfertile couples were examined. The median duratio n of infertility was 4.5 years (range 1-15) and the median age was 33 years (range 21-53). The outcome of AO staining ranged from 5 to 81%, with a median of 24%, green fluorescent spermatozoa, Results were not significantly related to the parameters of semen analysis (sperm count , motility, standard morphology, viability, pH and volume, as well as fructose concentration and number of round cells) or to local sperm an tibody testing and semen cultures, Fluorescence after AO staining was also not related to sperm functional capacity (evaluated using sperm-m ucus interaction tests in vitro and in vivo), or the medical history o f the patient, No significant differences in the AO test outcome were seen in patients with explained and unexplained infertility, or with r egard to subsequent fertility [with a median value of 21% (range 5-46) green fluorescence in the fertile group, compared with a median value of 28% (range 9-81) green fluorescence in the other men]. The results of this prospective study indicate that under the usual conditions of conception, the AO test is not clinically useful as a screening proce dure to determine semen quality during basic infertility investigation .