Method-of-moments estimators (MMEs) for the two-gene coefficients of r
elationship and inbreeding, and for the four-gene Cotterman coefficien
ts, are described. These estimators, which use co-dominant genetic mar
kers, are most appropriate for estimating pairwise relatedness or indi
vidual inbreeding coefficients, as opposed to their mean values in a g
roup. This is because, compared to the maximum likelihood estimate (ML
E), they show reduced small-sample bias and lack distributional assump
tions. The 'efficient' MME is an optimally weighted average of estimat
es given by each allele at each locus. Generally, weights must be comp
uted numerically, but if true coefficients are assumed zero, simplifie
d estimators are obtained whose relative efficiencies are quite high.
Population gene frequency is assumed to be assayed in a larger, 'refer
ence population' sample, and the biases introduced by small reference
samples and/or genetic drift of the reference population are discussed
. Individual-level estimates of relatedness or inbreeding, while displ
aying high variance, are useful in several applications as a covariate
in population studies.