I. Antonipillai et al., A 12-LIPOXYGENASE PRODUCT, 12-HYDROXYEICOSATETRAENOIC ACID, IS INCREASED IN DIABETICS WITH INCIPIENT AND EARLY RENAL-DISEASE, The Journal of clinical endocrinology and metabolism, 81(5), 1996, pp. 1940-1945
Earlier studies in diabetic animal models or ex vivo from diabetics su
ggest a deficiency in prostacyclin (PGI(2)) production and an increase
in an alternate arachidonic acid metabolite, 12-hydroxyeicosatetraeno
ic acid (18-HETE), which stimulates angiogenesis, mitogenesis, and inh
ibits renin secretion. We studied the urinary excretion rate of 6-keto
-PGF1(alpha) (a stable metabolite of PGI(2)) and 12-HETE in controls a
nd 42 noninsulin-dependent diabetes mellitus (NIDDM) patients with nor
mal renal function and those with micro- or macroalbuminuria/hyporenin
emic hypoaldosteronism (KH). The 2 eicosanoids were measured in wine u
sing previously described high pressure liquid chromatography and RIA
methods. Normal subjects and patients with NIDDM and microalbuminuria
were infused with low dose calcium infusions that stimulate prostacycl
in production in normal subjects. The PGI(2) excretion rate of NIDDM p
atients with normal renal function was not different from that of cont
rols (143 +/- 17 vs. 118 +/- 34 ng/g creatinine), but was reduced in t
hose with microalbuminuria (75 +/- 10) and in macroalbuminuria patient
s (48 +/- 7; P < 0.01). In contrast, 12-HETE was increased in diabetic
s with normal renal function as well as in those with micro- or macroa
lbuminuria patients (69 +/- 18 vs. 250 +/- 62 vs. 226 +/- 60 and 404 /- 131 ng/g creatinine; P < 0.01). Calcium did not stimulate PGI(2), b
ut increased 12-HETE in diabetics with microalbuminuria in contrast to
levels in normal subjects. HH patients excreted less PGI(2) (as previ
ously reported), but had increased 12-HETE. HETE/PGI(2) ratios further
demonstrated these changes in the various groups. In a nondiabetic hy
pertensive microalbuminuria group, 12-HETE excretion was normal (73 +/
- 28 ng/g creatinine). We conclude that the lipoxygenase product 12-HE
TE is increased early in the diabetic process, whereas PGI(2) producti
on is progressively impaired in NIDDM. These changes may play a role i
n the vascular disease of diabetes and partially explain the HH syndro
me.