CONSTRUCTION AND RAPID TESTING OF SYNTHETIC AND MODIFIED TOXIN GENE-SEQUENCES CRYIA (B-AND-C) BY EXPRESSION IN MAIZE ENDOSPERM CULTURE

Authors
SARDANA R DUKIANDJIEV S GIBAND M CHENG XY COWAN K SAUDER C ALTOSAAR I
Citation
R. Sardana et al., CONSTRUCTION AND RAPID TESTING OF SYNTHETIC AND MODIFIED TOXIN GENE-SEQUENCES CRYIA (B-AND-C) BY EXPRESSION IN MAIZE ENDOSPERM CULTURE, Plant cell reports, 15(9), 1996, pp. 677-681
Citations number
18
Categorie Soggetti
Plant Sciences
Journal title
Plant cell reports → ACNP
ISSN journal
07217714
Volume
15
Issue
9
Year of publication
1996
Pages
677 - 681
Database
ISI
SICI code
0721-7714(1996)15:9<677:CARTOS>2.0.ZU;2-4
Abstract
The synthesis of two modified genes, Cry IA(b) and CryIA(c), each cons isting of 1845 bp, is described in detail. The genes were synthesized using an improved PCR procedure based on recursive principles. The syn thetic CryIA(c) gene was put under the control of a maize ubiquitin pr omoter. This construct was tested in a maize endosperm-derived suspens ion culture system. The use of maize endosperm culture as a quick and efficient system to test the activity of synthetic genes is described.