Kj. Rao et T. Panda, EFFECT OF MODE OF OPERATION OF BIOREACTORS ON THE BIOSYNTHESIS OF PENICILLIN AMIDASE IN ESCHERICHIA-COLI, Bioprocess engineering, 14(6), 1996, pp. 317-321
Different operational mode of bioreactors influence the biosynthesis o
f the enzyme and related products as well. as the growth of industrial
microorganisms. This communication deals with the effect of mode of o
peration of various bioreactors with different geometric configuration
s, viz., batch (includes commercially available batch stirred tank, an
d custom-designed cylindrical and tapered reactors), batch-fed, contin
uous flow stirred tank reactors on the biosynthesis of penicillin amid
ase in Escherichia coli. Experimental findings show that the biosynthe
sis of penicillin amidase in E. coli show a little variation among bat
ch reactor modes and significant variation on the continuous mode of o
peration. Further analysis show that the different reactor modes also
influence periplasmic localization of the enzyme in the cell.