EFFECT OF MODE OF OPERATION OF BIOREACTORS ON THE BIOSYNTHESIS OF PENICILLIN AMIDASE IN ESCHERICHIA-COLI

Different operational mode of bioreactors influence the biosynthesis o f the enzyme and related products as well. as the growth of industrial microorganisms. This communication deals with the effect of mode of o peration of various bioreactors with different geometric configuration s, viz., batch (includes commercially available batch stirred tank, an d custom-designed cylindrical and tapered reactors), batch-fed, contin uous flow stirred tank reactors on the biosynthesis of penicillin amid ase in Escherichia coli. Experimental findings show that the biosynthe sis of penicillin amidase in E. coli show a little variation among bat ch reactor modes and significant variation on the continuous mode of o peration. Further analysis show that the different reactor modes also influence periplasmic localization of the enzyme in the cell.