TYPING OF VEROTOXINS BY DNA COLONY HYBRIDIZATION WITH POLYNUCLEOTIDE AND OLIGONUCLEOTIDE PROBES, A BEAD-ENZYME-LINKED IMMUNOSORBENT-ASSAY, AND POLYMERASE CHAIN-REACTION

Authors
YAMASAKI S LIN Z SHIRAI H TERAI A OKU Y ITO H OHMURA M KARASAWA T TSUKAMOTO T KURAZONO H TAKEDA Y
Citation
S. Yamasaki et al., TYPING OF VEROTOXINS BY DNA COLONY HYBRIDIZATION WITH POLYNUCLEOTIDE AND OLIGONUCLEOTIDE PROBES, A BEAD-ENZYME-LINKED IMMUNOSORBENT-ASSAY, AND POLYMERASE CHAIN-REACTION, Microbiology and immunology, 40(5), 1996, pp. 345-352
Citations number
34
Categorie Soggetti
Microbiology,Immunology
Journal title
Microbiology and immunology → ACNP
ISSN journal
03855600
Volume
40
Issue
5
Year of publication
1996
Pages
345 - 352
Database
ISI
SICI code
0385-5600(1996)40:5<345:TOVBDC>2.0.ZU;2-2
Abstract
To identify the type of Verotoxins (VT) produced by Verocytotoxin-prod ucing Escherichia coli (VTEC), a sensitive bead-enzyme-linked immunoso rbent assay and polymerase chain reaction with common and specific pri mers to various VTs (VT1, VT2, VT2vha, VT2vhb, and VT2vp1) were develo ped. Together with colony hybridization tests with oligo- and polynucl eotide probes, these methods were applied to VTEC isolates to type the VT produced. The toxin types of 26 of 37 strains were identified, but the reaction profiles in assays of the remaining 11 strains suggested the existence of new VT2 variants. The application of these identific ation procedures may be useful as a tool for clinical and epidemiologi cal studies of VTEC infection.