S. Benyoucef et al., A MICROASSAY FOR DETERMINATION OF THE CYTOPATHOGENICITY OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ISOLATES, Microbiology and immunology, 40(5), 1996, pp. 381-388
Correlations between the in vitro biological properties of HIV strains
isolated from patients and the prognosis of their disease have been r
eported. We developed a technique to study the phenotype of HIV strain
s isolated from patients. We used the P4 cell line, derived from HeLa
cells, which has been transfected with receptor CD4 gene. HIV laborato
ry strain (HIVLAI) and peripheral blood leukocytes (PBLs) from donors
infected with HIVLAI induce syncytium in P4 cell cultures in vitro. Th
e presence of reporter gene (LacZ gene) under the control of the HIV-1
long terminal repeat (LTR) in these cells allows colorimetric visuali
zation of syncytia in the cytoplasm using a beta-galactosidase (beta g
al) assay in the presence of X-gal. We cocultivated 1x10(6) patient PB
Ls with 2 x 10(6) normal PHA-activated normal PBLs for 4 days in the p
resence of IL-2 in 24-well plates. Half of the medium was replaced twi
ce a week and PHA-activated normal PBLs were added every 7 days. HIV-1
was isolated from cocultured PBLs of 18 patients with advanced-stage
HIV infection as assessed by the production of HIV p24 detected with a
commercially available HIV-1 p24 ELISA. Supernatant and 10(5) cells w
ere collected twice a week from cocultured PBLs and were added to P4 c
ells in 96-well microtiter plates. The cultures were observed every da
y for 3 days and then the beta gal assay was performed. We did not obs
erve any effect with cells and supernatant from 8 patients, harvested
from cultures incubated for as long as 28 days. The phenotype of these
isolates was called NC (noncytopathic). In cells from 2 patients, we
obtained blue multinucleated giant cells; the phenotype of these strai
ns was called SI (syncytium inducing). In cultures from 8 other patien
ts, we obtained the death of P4 cells without syncytium formation, and
the phenotype of these strains was called CI (cell-killing inducing).
In every case, the cytopathic effect of HIV-1 isolates could be detec
ted with cocultured PBLs collected as early as day 4 of culture. Cocul
tured PBLs from 13 healthy controls did not alter the P4 cells. We dis
played the replication of CI strains of HIV-1, but not the one of NC s
trains in P4 cell line, Our micromethod allowed the detection of cytop
athic effects of HIV isolates. Further investigations should define th
e clinical applications of this method.