AMINO-ACID-RESIDUES AFFECTING THE ACTIVITY AND STABILITY OF HUMAN O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE

Amino acid residues in the human O-6-alkylguanine-DNA alkyltransferase (AGT) were mutated and seventeen of the mutant proteins expressed in the ada(-) ogt(-) E. coli strain GWR 109 which is very sensitive to ki lling by methylating agents because of the absence of endogenous alkyl transferases. Thirteen of the mutations tested (Delta 10, Delta 1-19, R128A, N137A, H146A, R147A, Delta N157, Y158A, E172Q, Delta 92-97, Y11 4E, C145A and E172stop) reduced activity to below detectable levels wh en crude cell extracts were tested for the ability to remove O-6-[H-3] methylguanine from H-3-methylated DNA. However, only 4 of these mutati ons (Delta 92-97, Y114E, C145A and E172stop) led to a complete loss of activity when tested for the ability to protect the cells from killin g by MNNG. This suggests that the other nine mutations do not lead to the complete inactivation of AGT but produce protein with a reduced ac tivity or in reduced amounts. These results show that none of the resi dues altered in these mutations (Delta 1-10, Delta 1-19, R128A, N137A, Delta N157, H146A, R147A, Y158A and E172Q) are absolutely essential f or AGT activity in protection against killing by MNNG. The stability o f the mutant AGT proteins was determined by measuring the half-life of the protein synthesis was blocked. These results indicated that five of mutants that lacked AGT activity when tested in the crude extracts (Y114E, R128A, C145A, Delta N157 and Y158A) were stable in the cell sh owing that the alteration of these residues does greatly reduce AGT ac tivity. The other eight mutants lacking activity in crude extracts (De lta 1-10, Delta 92-97, E172Q, E172stop, Delta 1-19, N137A, H146A and R 147A) produced a large decrease in the stability of the AGT protein. T his may account for the inability to detect AGT activity in vitro desp ite the ability to protect fr om MNNG toxicity in vivo. It is of parti cular interest that mutation of residues His(146), Arg(147), Asn(137) and Glu(172) resulted in unstable AGT proteins active in vivo but not in vitro. The crystal structure of the related Ada-C alkyltransferase suggests the involvement of these residues with the Cys(145) acceptor site in a hydrogen bond network that may stabilize the protein and aid in the reaction mechanism. The data presented here support the existe nce of such an interaction existing in the human AGT and stress its im portance in maintaining the configuration of the protein.