SOME PRACTICAL ASPECTS OF DOUBLE-RESONANCE TECHNIQUES IN SOLUTION-STATE NMR-STUDIES OF HIGH-MOLECULAR-WEIGHT SYSTEMS

This paper addresses some of the practical issues associated with solu tion NMR investigations of high-molecular-weight (>20 kDa) biomolecule s and their complexes. The experimental example of a high-molecular-we ight system used here is a 52 kDa Fab/peptide complex. Theoretical as well as experimental aspects of solution NMR techniques employed to st udy such larger systems are discussed. Specific topics covered include hardware considerations, solvent suppression, and the relative effici encies of various isotope-edited NMR techniques, Typical NOE methods u sed to derive structural information on these higher-molecular-weight systems and the scope of structural detail achievable are also examine d. (C) 1996 Academic Press, Inc.