FUNCTIONAL EXPRESSION OF THE EXTRAPLASTIDIAL ARABIDOPSIS-THALIANA OLEATE DESATURASE GENE (FAD2) IN SACCHAROMYCES-CEREVISIAE

Authors
COVELLO PS REED DW
Citation
Ps. Covello et Dw. Reed, FUNCTIONAL EXPRESSION OF THE EXTRAPLASTIDIAL ARABIDOPSIS-THALIANA OLEATE DESATURASE GENE (FAD2) IN SACCHAROMYCES-CEREVISIAE, Plant physiology, 111(1), 1996, pp. 223-226
Citations number
20
Categorie Soggetti
Plant Sciences
Journal title
Plant physiology → ACNP
ISSN journal
00320889
Volume
111
Issue
1
Year of publication
1996
Pages
223 - 226
Database
ISI
SICI code
0032-0889(1996)111:1<223:FEOTEA>2.0.ZU;2-J
Abstract
The functional expression in yeast of the Arabidopsis thaliana FAD2 ge ne, encoding the extraplastidial oleate desaturase (1-acyl-2-oleoyl-sn -glycero-3-phosphocholine Delta 12-desaturase) is reported. Dienoic fa tty acids constituted up to 11% (w/w) of the total fatty acids in tran sformed Saccharomyces cerevisiae cells and were confirmed to be linole ic acid and Delta(9), Delta(12)-hexadecadienoic acid by gas chromatogr aphy-mass spectrometry.