H. Masoud et Mb. Perry, STRUCTURAL CHARACTERIZATION OF THE O-ANTIGENIC POLYSACCHARIDE OF ESCHERICHIA-COLI SEROTYPE O-17 LIPOPOLYSACCHARIDE, Biochemistry and cell biology, 74(2), 1996, pp. 241-248
The structure of the O-polysaccharide component of the lipopolysacchar
ide produced by Escherichia coli 017 (ATCC 23512) was determined by th
e use of methylation, periodate oxidation, one- and two-dimensional nu
clear magnetic resonance spectroscopy, and mass spectrometric methods.
The O-polysaccharide was found to be a high molecular weight polymer
of repeating branched pentasaccharide units composed of D-mannose, D-g
lucose, and 2-acetamido-2-deoxy-D-glucose residues (3:1:1) and had the
structure [GRAPHICS] arbohydrate units. The isolated glycoconjugate s
tained ''blue'' with Stains All and migrated as a single band on sodiu
m dodecyl sulfate gradient gels (2.5-10% acrylamide - diallyl tartardi
amide) at an estimated molecular weight of 540 000. Amino acid and ami
no sugar analyses showed that it is rich in aspartic acid - asparagine
, glutamic acid - glutamine, alanine, proline, and glycine, and contai
ns galactosamine and glucoasmine. This transforming growth factor-beta
inducible glycoprotein may be involved in cell differentiation and in
the cartilage repair process. It may also be used as a marker to loca
lize the biological activity of transforming growth factor-beta in art
icular cartilage.