THE USE OF RAPD TECHNIQUE FOR THE IDENTIFICATION AND CLASSIFICATION OF PISUM-SATIVUM L GENOTYPES

The genomic DNAs of 42 Pisum sativum genotypes, representing four wild and cultivated subspecies were used as templates in RAPD reactions. A mplification with eight decamer primers generated 149 polymorphic prod ucts. Genetic similarities of RAPD profiles were estimated via a coeff icient of Jaccard and then the data were processed by cluster analysis (UPGMA). Each genotype was clearly identified and separated from the others. Our results show that RAPD technology is a rapid, precise and sensitive technique for identification of pea genotypes. However, the phylogenetic relationships within the Pisum sativum, which we tested b y bootstrap analysis (Wagner parsimony), must be interpreted with caut ion.