HORMONAL AND NUTRITIONAL MODULATION OF HEPATIC ARGINASE ACTIVITY IN GROWING CATTLE

The hormonal and nutritional modulation of hepatic arginase activity ( HARG) was characterized in growing cattle in two studies. In the first study, 20 steers (initial weight, 182 +/- 2 kg) were assigned in equa l numbers to either Synovex-S (SYN) (ear implant), recombinant bovine somatotropin (Somavubove; SbV; 0.1 mg/kg intramuscularly daily), SYN SbV, or nothing (control). Steers were individually fed, for 56 d, a concentrate (80% diet dry matter [DM]) and silage (20% diet DM) diet p roviding 20 g of crude protein (CP) and 252 kcal metabolizable energy (ME) per kg body weight(0.75). On Day 57, samples of liver were obtain ed at slaughter and subsequently assayed for HARG by the incubation of a tissue homogenate for 2 hr with 250 mM arginine, with and without M n2+ and heat activation, and the measurement of the resulting urea. HA RG was uniformly increased by divalent cation (Mn2+) and heating. SYN had no effect on HARG, whereas SbV treatment resulted in an overall 34 % decrease in HARG. Plasma urea nitrogen (PUN) was decreased by SbV bu t not consistently affected by SYN. In the second study, 16 steers (av g. initial weight, 284 +/- 5 kg) were initially fed a concentrate basa l diet consisting of 11% CP and 1.96 Meal ME for 21 d. Steers were the n assigned to one of four dietary treatments (6.4 kg DM/hr per day) in a factorial arrangement of high and low CP (8 and 14%) and two levels of energy (1.96 and 2.67 Meal ME/kg of diet DM) for 210 d and slaught ered. HARG and PUN were higher in steers fed 14% CP but were lower at each level of CP fed at the higher level of ME. The data suggest that hormonal repartitioning compounds and diet composition may modulate ni trogen metabolism by affecting the activity of arginase in the liver a s well as by affecting the total content of arginase in association wi th changes in organ size.