Hole-burning and fluorescence-line-narrowing (FLN) spectroscopies are
used to generate high-resolution vibronic spectra of TO-PRO-3 iodide i
n a glycerol/water glass. The excited-state vibrational frequencies ar
e tabulated along with the associated Franck-Condon factors (FCFs). It
is also shown that hole-burning efficiencies are comparable for the f
ree dye and the dye bound to DNA. (C) 1996 John Wiley & Sons, Inc.