NATURAL-KILLER-CELL ACTIVITY IN CELIAC-DISEASE - EFFECT OF IN-VITRO TREATMENT ON EFFECTOR LYMPHOCYTES AND OR TARGET LYMPHOBLASTOID, MYELOIDAND EPITHELIAL-CELL LINES WITH GLIADIN/
Maf. Castany et al., NATURAL-KILLER-CELL ACTIVITY IN CELIAC-DISEASE - EFFECT OF IN-VITRO TREATMENT ON EFFECTOR LYMPHOCYTES AND OR TARGET LYMPHOBLASTOID, MYELOIDAND EPITHELIAL-CELL LINES WITH GLIADIN/, Folia microbiologica, 40(6), 1995, pp. 615-620
To analyze the possible involvement of natural killer (NK) cell activi
ty in the pathogenetic mechanism of coeliac disease (CD) we measured t
he spontaneous cytotoxic cell activity of peripheral blood mononuclear
cells (PMNC) from patients with CD and from healthy donors. No signif
icant differences were found between the NK cell activity of PMNC from
healthy donors and from patients with CD using a standard Cr-51 relea
se assay. However, a 30-min treatment of PMNC with gliadin inhibited N
K cell activity in patients with CD. On the other hand, a 1-d incubati
on with gliadin induced cytotoxic cell activity of PMNC against the NK
-resistant target cells such as the epithelial HT-29 and the lymphobla
stoid RNJI cell lines, suggesting that activation of PMNC by cultivati
on with gliadin can occur.