CHARACTERIZATION OF HUMAN, MOUSE AND RABBIT ANTIGLIADIN ANTIBODIES BYELISA AND WESTERN BLOTTING

Monoclonal, hyperimmune rabbit and human serum anti-gliadin antibodies were analyzed by ELISA and immunoblotting techniques. In Western blot ting the difference in reactivity between monoclonal and human antibod ies was quantitative rather than qualitative. Rabbit antisera differed in reactivity according to the protein used for immunization. The rab bits immunized by the peptic-tryptic pancreatic digest of gliadin reac ted similarly to the patients. In ELISA, significantly higher reactivi ty with crude, A-, glyc-gli, alpha-, beta- and omega-gliadins was foun d in the patients' sera than in controls.