COMPARISON OF VIRULENCE OF DIFFERENT ACTINOBACILLUS-PLEUROPNEUMONIAE SEROTYPES AND BIOTYPES USING AN AEROSOL INFECTION MODEL

Citation
Mj. Jacobsen et al., COMPARISON OF VIRULENCE OF DIFFERENT ACTINOBACILLUS-PLEUROPNEUMONIAE SEROTYPES AND BIOTYPES USING AN AEROSOL INFECTION MODEL, Veterinary microbiology, 49(3-4), 1996, pp. 159-168
Citations number
20
Categorie Soggetti
Microbiology,"Veterinary Sciences
Journal title
ISSN journal
03781135
Volume
49
Issue
3-4
Year of publication
1996
Pages
159 - 168
Database
ISI
SICI code
0378-1135(1996)49:3-4<159:COVODA>2.0.ZU;2-5
Abstract
An aerosol infection model for inoculation of pigs with Actinobacillus pleuropneumoniae is described, With this model the virulence of three A. pleuropneumoniae biotype 1 strains representing serotypes 2, 5b an d 6, and one Danish biotype 2 were compared using 13-week-old pigs for inoculation. The pigs were sacrificed 24 h after aerosol exposure and lung lesions were evaluated. In pigs exposed to aerosols of suspensio ns containing 10(4) CFU/ml of serotypes 2, 5b and 6, a number of 5-10 lesions of haemorrhagic necrotizing pneumonia were induced, For the bi otype 2 strain the dose creating similar lesions was 10(9) CFU/ml. Rep eated experiments confirmed these results showing similar virulence of serotypes 2, 5b and 6 whereas the biotype 2 strain proved less virule nt, The aerosol infection model allowed a comparison of the number of A. pleuropneumoniae CFU/liter air which were necessary to induce lung lesions in susceptible pigs, This indicates that the model will be wel l suited for virulence studies of A. pleuropneumoniae serotypes in pig s.