The difficulties in developing inter-varietal chromosome substitution
lines in wheat are reviewed. The use of genetical, cytological and mol
ecular markers is suggested as a way of overcoming them. These difficu
lties and the use of markers, as well as the need to develop duplicate
lines to detect background variation, are described using the develop
ment and analysis of the Cappelle-Desprez (Bezostaya 1) chromosome sub
stitution set as an example. The effects of substituting Bezostaya 1 c
hromosomes on final plant height and adult-plant resistance to yellow
rust are reported. The large number of aneuploids and substitution lin
es available in wheat provides a tremendous, international resource, w
hich should be exploited in the future.