THE USE OF WHEAT ANEUPLOIDS FOR THE CHROMOSOMAL ASSIGNMENT OF MICROSATELLITE LOCI

The chromosomal assignment of 64 PCR-amplified microsatellite loci and 29 additional fragments amplified by the same primer pairs is describ ed for bread wheat (Triticum aestivum). The distribution over the diff erent chromosomes and chromosome arms appears to be random. The highes t proportion of microsatellite loci is found on the B genome, followed by the A and D genome. About half of the primer pairs amplified uniqu e fragments, while the other half amplified additional fragments. 25% of the primer pairs, mostly designed to clones of a PstI-library, ampl ify fragments on homoeologous chromosomes. In some cases, more than on e fragment on a single chromosome or fragments on non-homoeologous chr omosomes occurred. The use of an automated DNA sequencer accounts for the accurate resolution of multiple fragments and enables to different iate between fragments, amplified by a single primer pair, with size d ifferences as small as two base pairs.