CYTOLOGICAL ANALYSIS OF PLANT CHROMOSOMES USING RAPID IN-SITU HYBRIDIZATION

A rapid fluorescent in situ hybridization protocol, which can be compl eted in one working day, has been utilised to visualize chromosome pre parations of barley, chickpea, white clover, millet, rye, sugarcane, w heat, Aegilops spp. and Leymus spp. Both total genomic DNA and cloned nuclear rDNA were used as a probe. This protocol has the advantage of brevity and flexibility over more conventional approaches, and has mad e fluorescent in situ hybridization a routine cytological procedure.