EFFECT OF LITHOCHOLATE-3-SULFATE AND ITS AMIDES ON BILIARY-EXCRETION OF GLUTATHIONE AND PHOSPHOLIPID

Some organic anions and lithocholate-3-O-glucuronide have been reporte d to inhibit biliary glutathione excretion. In this study the effect o f lithocholate-3-sulfate on biliary glutathione excretion was examined in rats. Furthermore, the effects of its amides on biliary glutathion e and phospholipid excretion were also studied. Intravenously injected lithocholate-3-sulfate (3 mu mol/100 g body wt.) depressed biliary gl utathione excretion to 15% of the basal value, which corresponded to b iliary lithocholate-3-sulfate excretion. The same dose of glyco- and t aurolithocholate-3-sulfate also inhibited biliary glutathione excretio n, and its inhibition was more marked with glycolithocholate-3-sulfate . In contrast, taurolithocholate-3-sulfate increased the bile acid/pho spholipid ratio in the bile more markedly than glycolithocholate-3-sul fate did. These findings indicate that the mechanisms for inhibition b y lithocholate-3-sulfate amides of biliary excretion of glutathione an d phospholipid are different.