CYTOTOXIC T-LYMPHOCYTES, MEMORY CELLS, AND T-CELL SUPPRESSORS SPECIFIC TO ALLOANTIGEN H-2K(B) DIFFER IN RECOGNITION OF MHC MOLECULE H-2K(B)PEPTIDES

Six individual peptides of H-2K(b) molecule (MHC class I) correspondin g to variable sequences of alpha(1) domain (peptides 1 and 2) and alph a(2) domain (peptides 3-6) were selected. Conditions suppressing T-lym phocyte CD8(+) (CTL, mCTL, mCTL-2, and Ts) nonspecific adhesion on syn geneic macrophage monolayers were determined. Using adsorption-elution on syngeneic macrophage monolayers pretreated with peptides, it was s hown that effector CTL do not interact with peptides of MHC class I mo lecules, Ts interact with alpha(2) domain peptides (peptides 4, 5, and 6), and mCTL interact with peptide 4 of alpha(2) domain. Hence the re sults obtained provide the basis for selective induction of different T-lymphocyte subclasses by MHC molecule peptides.