THERMODYNAMIC ANALYSIS OF THE INTERACTION OF ETHIDIUM-BROMIDE WITH TETRADEOXYRIBONUCLEOTIDE 5'-D(GPCPGPC) USING H-1-NMR DATA

Authors
VESELKOV AN DYMANT LN BOLOTIN PA BARANOVSKII SF VESELKOV DA SHIPP D DAVIES D
Citation
An. Veselkov et al., THERMODYNAMIC ANALYSIS OF THE INTERACTION OF ETHIDIUM-BROMIDE WITH TETRADEOXYRIBONUCLEOTIDE 5'-D(GPCPGPC) USING H-1-NMR DATA, Molecular biology, 30(1), 1996, pp. 111-116
Citations number
24
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
30
Issue
1
Year of publication
1996
Part
2
Pages
111 - 116
Database
ISI
SICI code
0026-8933(1996)30:1<111:TAOTIO>2.0.ZU;2-3
Abstract
Temperature dependences of proton chemical shifts for ethidium bromide in aqueous solution with tetradeoxyribonucleotide 5'-d(GpCpGpC) were studied by 500-MHz pulse NMR spectrometry. Calculation of the thermody namic parameters of complexation yielded estimates for contributions o f reactions giving rise to different types of complexes under complex equilibrium conditions. Gibbs energies, enthalpies, and entropies were determined for the formation of 1:1, 2:1, 1:2, and 2:2 complexes betw een EthBr and GCGC.