DEMONSTRATION OF THE OLIGOCLONALITY OF AN ENTEROPATHY-ASSOCIATED T-CELL LYMPHOMA BY MONOCLONAL-ANTIBODIES AND PCR ANALYSIS OF THE T-CELL RECEPTOR V-BETA REPERTOIRE ON FIXED TISSUE

The apparent clonality of T cells present in enteropathy associated T cell lymphomas (EATCLs) has previously been reported by showing T cell receptor (TCR) gene rearrangement in fresh tumor tissue. The EATCL pr esented here exhibits the novel phenotype CD3(+), HML-1(+), CD4(+), CD 8(+), and TCR V beta 8(+). The oligoclonality of the tumor cells is sh own using the polymerase chain reaction (PCR) on cDNA from RNA extract ed from formalin-fixed paraffin-embedded tissue. The T cells present i n the lymphoma were predominantly TCR V beta 8(+). (C) 1996 by W.B. Sa unders Company