PLASMALOGENS AND THEIR OXIDATIVE-DEGRADATION PRODUCTS IN LOW AND HIGH-DENSITY-LIPOPROTEIN

The kinetics of the autoxidation reaction of LDL and HDL with Fe2+/asc orbate was investigated. Aldehydes derived from plasmalogens were dete rmined by conversion to 2-alkyl-1,3-dithiolanes. Their oxidation produ cts, 2-hydroxy-aldehydes, were trapped by reaction with pentafluoroben zylhydroxylamine. After derivatisation, the pentafluorobenzyloximes co uld be separated from other compounds by thin-layer chromatography. Th ey were detected by gas chromatography applying an electron capture de tector. LDL plasmalogens are oxidized nearly completely after 180 min while HDL plasmalogens suffer oxidation only to 5%. Oxidation of linol eic and arachidonic acid was investigated by simultaneous determinatio n of 2-hydroxyheptanal.