BLOOD MICROCIRCULATION AND IMMUNE-RESPONS E OF CELLS IN REGIONAL LYMPH-NODES IN CANCER - IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL-STUDY

Immunohistochemical, ultrastructural, morphological and morphometric t echniques as well as monoclonal antibodies to F VIII R:Ag endotheliocy tes, CD2 and CD3 T-lymphocytes, CD1 and CD10 T-lymphoblasts, CD4 T hel pers, CD8 and CD30 T-killers and activated lymphocytes. were used to s tudy blood microcirculation relationship to cellular immunity in lymph nodes regional to malignant tumor. It was established that on the one hand there was early activation of cellular immune reactions with hig h level of cytotoxic T-cells and enhanced lymphocyte recirculation via postcapillary venules, on the other side at later stages changes in t he vascular wall and circulatory disorders develop in line with deposi tion of intra- and extravascular fibrin. This impedes lymphocyte recir culation and leads to a cut in the number of T-effectors enabling prev ention of metastatic spread to the lymph nodes.