CAPILLARY ELECTROPHORESIS WITH WAVELENGTH-RESOLVED FLUORESCENCE DETECTION

Single-channel fluorescence detectors provide extremely low limits of detection for analytes separated by capillary electrophoresis (CE), bu t little structural or diagnostic information. In wavelength-resolved fluorescence detection, complete fluorescence emission spectra are acq uired for every analyte separated by CE, The fluorescence spectral inf ormation has been used for DIVA sequencing and to identify the tyrosin e and tryptophan content of peptides. In addition, a variety of diagno stic uses for the fluorescence information exist, including monitoring excitation source stability and the pH, organic content and impuritie s in the running buffer. The popularity of spectrally resolved fluores cence detection in CE is expected to increase just as diode-an ay dete ction has become relatively common for ultraviolet-visible absorbance.