HUMAN MONOCLONAL-ANTIBODY AGAINST COLON-CANCER

The purpose of the study was to produce human monoclonal antibodies (h McAb) against human colon cancer for use in radioimmunoimaging. Human- mouse heterohybridomas were developed by fusing SHM-D33 mouse-human hy brid heteromyeloma cells with human lymphocytes from colon cancer tumo r-draining lymph nodes. The hybridomas capable of secreting human mono clonal antibodies were screened by using human colon cancer cell lines and pathological biopsies with ELISA and immunohistochemical methods, hMcAb clone H11 was selected for a large-scale antibody production, w hich was purified from mouse ascites. Biodistribution study demonstrat ed that specific uptake of I-125-hMcAb H11 by human colon cancer xenog rafts was significantly higher than by normal tissues. Radioimmunoimag ing of human colon cancer xenografts exhibited distinct tumor visualiz ation during the period of 72-96 hr after intraperitoneal injection of I-125-hMcAb H11. The development of human monoclonal antibodies such as hMcAb H11 may be useful for radioimmunodetection and therapy of col on cancer.