The products of condensation of organic alcohols and nitric acid are n
itrate esters with the general structure C-O-NO2, These products are w
idely employed as vasodilators and explosives, and are true xenobiotic
compounds, as they do not occur in nature, We have isolated and chara
cterized a consortium of two microorganisms, Arthrobacter ilicis and A
grobacterium radiobacter, that mineralized recalcitrant ethylene glyco
l dinitrate. The Arthrobacter strain was the actual degrading microorg
anism, although the second microbe facilitated mineralization. The bio
degradation of ethylene glycol dinitrate by A. ilicis involved the pro
gressive elimination of the nitro groups from the organic molecule to
generate ethylene glycol, which was then mineralized, Waters polluted
with ethylene glycol dinitrate have been shown amenable to biological
treatment in a pilot plant with wastewaters generated during the synth
esis of the chemical in a factory.