GENETIC IDENTIFICATION OF CRYPTIC GENOSPECIES OF HAEMOPHILUS CAUSING UROGENITAL AND NEONATAL INFECTIONS BY PCR USING SPECIFIC PRIMERS TARGETING GENES-CODING FOR 16S RIBOSOMAL-RNA
R. Quentin et al., GENETIC IDENTIFICATION OF CRYPTIC GENOSPECIES OF HAEMOPHILUS CAUSING UROGENITAL AND NEONATAL INFECTIONS BY PCR USING SPECIFIC PRIMERS TARGETING GENES-CODING FOR 16S RIBOSOMAL-RNA, Journal of clinical microbiology, 34(6), 1996, pp. 1380-1385
Previous genetic analysis of Haemophilus influenzae strains isolated f
rom genital and neonatal infections identified a group of biotype IV t
hat constitutes a cryptic genospecies only distantly related to H. inf
luenzae and H. haemolyticus. Small-subunit rRNA genes of two represent
ative strains of this genital Haemophilus genospecies (strains 16N and
2406) were sequenced. The analysis indicated that these strains form
a monophyletic unit with H. haemolyticus and H. influenzae biogroups.
Influenzae and Aegyptius and are more closely related to H. haemolytic
us than to H. influenzae biogroups Influenzae and Aegyptius. 16S rRNA
gene sequences were used to formulate primers for PCR-based identifica
tion of cryptic genital Haemophilus organisms. A 242-bp fragment was a
mplified from strains belonging to the genital Haemophilus genospecies
but not from strains of 12 other Haemophilus species, including strai
ns of H. influenzae biotype IV sensu stricto.