GENETIC IDENTIFICATION OF CRYPTIC GENOSPECIES OF HAEMOPHILUS CAUSING UROGENITAL AND NEONATAL INFECTIONS BY PCR USING SPECIFIC PRIMERS TARGETING GENES-CODING FOR 16S RIBOSOMAL-RNA

Citation
R. Quentin et al., GENETIC IDENTIFICATION OF CRYPTIC GENOSPECIES OF HAEMOPHILUS CAUSING UROGENITAL AND NEONATAL INFECTIONS BY PCR USING SPECIFIC PRIMERS TARGETING GENES-CODING FOR 16S RIBOSOMAL-RNA, Journal of clinical microbiology, 34(6), 1996, pp. 1380-1385
Citations number
28
Categorie Soggetti
Microbiology
ISSN journal
00951137
Volume
34
Issue
6
Year of publication
1996
Pages
1380 - 1385
Database
ISI
SICI code
0095-1137(1996)34:6<1380:GIOCGO>2.0.ZU;2-A
Abstract
Previous genetic analysis of Haemophilus influenzae strains isolated f rom genital and neonatal infections identified a group of biotype IV t hat constitutes a cryptic genospecies only distantly related to H. inf luenzae and H. haemolyticus. Small-subunit rRNA genes of two represent ative strains of this genital Haemophilus genospecies (strains 16N and 2406) were sequenced. The analysis indicated that these strains form a monophyletic unit with H. haemolyticus and H. influenzae biogroups. Influenzae and Aegyptius and are more closely related to H. haemolytic us than to H. influenzae biogroups Influenzae and Aegyptius. 16S rRNA gene sequences were used to formulate primers for PCR-based identifica tion of cryptic genital Haemophilus organisms. A 242-bp fragment was a mplified from strains belonging to the genital Haemophilus genospecies but not from strains of 12 other Haemophilus species, including strai ns of H. influenzae biotype IV sensu stricto.