DETECTION OF ENTEROVIRUSES FROM CLINICAL SPECIMENS BY SPIN AMPLIFICATION SHELL VIAL CULTURE AND MONOCLONAL-ANTIBODY ASSAY

Conventional tube cell culture was compared with a 72-h, spin-amplifie d shell vial indirect immunofluorescence assay for the detection of en terovirus from clinical specimens. The sensitivity and specificity for the shell vial assay after resolution of discrepant results were 93 a nd 100%, respectively. The shell vial assay detected 93% of the positi ve cultures within 72 h of incubation while conventional tube culture detected only 51% of the positive cultures within the same time interv al. The data suggest that a spin-amplified shell vial indirect immunof luorescence assay may be useful for the detection of enterovirus from clinical specimens.