EVALUATION OF PULSED-FIELD GEL-ELECTROPHORESIS AS A TYPING SYSTEM FORCANDIDA-RUGOSA - COMPARISON OF KARYOTYPE AND RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS

Citation
Jc. Dib et al., EVALUATION OF PULSED-FIELD GEL-ELECTROPHORESIS AS A TYPING SYSTEM FORCANDIDA-RUGOSA - COMPARISON OF KARYOTYPE AND RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS, Journal of clinical microbiology, 34(6), 1996, pp. 1494-1496
Citations number
25
Categorie Soggetti
Microbiology
ISSN journal
00951137
Volume
34
Issue
6
Year of publication
1996
Pages
1494 - 1496
Database
ISI
SICI code
0095-1137(1996)34:6<1494:EOPGAA>2.0.ZU;2-L
Abstract
Nosocomial infections with Candida species have emerged as an increasi ngly important cause of morbidity and mortality in intensive care unit s. Ten Candida rugosa isolates from a previously documented cluster of C. rugosa infections in one hospital (nine burn unit isolates and one isolate from another hospital ward) and eight C. rugosa isolates reco vered in a referral fungus testing laboratory (comparison isolates) fr om distinct geographic areas were investigated by molecular techniques . Isolates were from multiple anatomic sites. Pulsed-field gel electro phoresis (PFGE) of whole-cell DNA was performed with the 18 C, rugosa isolates as a marker of strain identity. The PFGE karyotypes of the C. rugosa isolates were demonstrated from four to seven chromosome bands . Karyotyping revealed the same PFGE pattern for the nine outbreak iso lates from the burn unit, confirming clonal strain transmission. The i solate from the other hospital ward had a distinct karyotype, Distinct PFGE karyotype patterns were demonstrated for the eight comparison is olates. Restriction fragment length polymorphisms (RFLP) generated fro m whole-cell DNA digested with SfiI demonstrated the same RFLP pattern among outbreak isolates. Among comparison isolates, karyotyping disti nguished some isolates that were indistinguishable by RFLP patterns, K aryotyping by PFGE appears to be the most useful molecular typing tool for discrimination among strains of C. rugosa and will be a useful ma rker for evaluating the epidemiology of future C. rugosa infections.