DETECTION OF HEPATITIS-E VIRUS-RNA IN STOOLS AND SERUM BY REVERSE TRANSCRIPTION-PCR

Authors
TURKOGLU S LAZIZI Y MENG JH KORDOSI A DUBREUIL P CRESCENZO B BENJELLOUN S NORDMANN P PILLOT J
Citation
S. Turkoglu et al., DETECTION OF HEPATITIS-E VIRUS-RNA IN STOOLS AND SERUM BY REVERSE TRANSCRIPTION-PCR, Journal of clinical microbiology, 34(6), 1996, pp. 1568-1571
Citations number
8
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
34
Issue
6
Year of publication
1996
Pages
1568 - 1571
Database
ISI
SICI code
0095-1137(1996)34:6<1568:DOHVIS>2.0.ZU;2-1
Abstract
Stools and sera collected during an experimental hepatitis E virus (HE V) infection in monkeys and collected from humans with acute HEV infec tions during epidemic and sporadic cases were analyzed by reverse tran scription-PCR. Two methods for RNA purification were compared. Protein ase K digestion and phenolchloroform extraction were more efficient th an guanidinium isothiocyanate extraction in improving the sensitivity and specificity for the detection of HEV genomes.