ENHANCED AMPLIFICATION OF HEPATITIS-C VIRUS (HCV) CDNA BY PCR - DETECTION OF HCV RNA IN ARCHIVAL SERA

Authors
BEARDSLEY AM GOWANS EJ BURRELL CJ MARMION BP
Citation
Am. Beardsley et al., ENHANCED AMPLIFICATION OF HEPATITIS-C VIRUS (HCV) CDNA BY PCR - DETECTION OF HCV RNA IN ARCHIVAL SERA, Journal of clinical microbiology, 34(6), 1996, pp. 1581-1582
Citations number
16
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
34
Issue
6
Year of publication
1996
Pages
1581 - 1582
Database
ISI
SICI code
0095-1137(1996)34:6<1581:EAOHV(>2.0.ZU;2-4
Abstract
A reverse transcription-PCR assay which successfully amplified hepatit is C virus RNA from poorly stored archival sera was optimized. Maximum sensitivity was achieved with Moloney murine leukemia virus RNase H- reverse transcriptase and by a single round of PCR amplification of a short (112-bp) fragment of the 5' untranslated region of the viral gen ome.