The karyotype of stoneloach, Noemacheilus barbatulus from Utrata river
was studied by-conventional Giemsa staining, Ag-NOR technique and C-b
anding. The diploid chromosome number 2n = 50, was composed of 4 pairs
of metacentric, 10 pairs of submetacentric and 11 pairs of subtelo- t
o acrocentric chromosomes (NF = 78). The NORs bearing chromosomes were
from the biggest metacentric pair and they were-located on terminal p
osition. This region was also positive for C-banding. The massive hete
rochromatin block was also localized on the arms, on one side of centr
omer, on the second pair of metacentric chromosomes. Almost all other
biarmed and uniarmed elements of chromosome set had C-positive heteroc
hromatin in the centromers. Most of the species studied from subfamily
Noemacheilinae had diploid chromosomes number 2n = 50. In comparison
with the fish from subfamily Cobitidinae a lack of interpopulation chr
omosomal variability was observed. The results of karyotype of Noemach
eilus barbatulus in comparison with the previously conducted studies a
re thus suggesting karyological stability.